You betcha, KOHO can be used to rent cars! Though not all franchisees will accept pre-paid Visa cards. From experience, we know that the following merchants do: Enterprise (depending on the location), Alamo, Hertz (depending on the location) and Advantage.
Just a heads up that for car rentals, hotels, and gas stations, merchants may put a pre-authorization hold (usually around 15-20%) on your card. This is by no means a charge, however, your funds will be held to the ensure you have enough to cover the purchase (plus incidentals where applicable). The correct amount will ‘settle’ within 15 business days after the purchase.
Hot Tip: to get around this hassle, book your rental car using a different credit card and then pay the final amount using your KOHO card. Happy Travels 🚘